Summary quantitative pcr is a method used to detect relative or absolute gene expression level. Qpcr sybr green mix refer to qpcr selection guides part 1 and part 2 dna cdna template cdna reaction diluted 1 10 to detect a medium to highly expressed targets or 1 2 to 1 5 for rare transcripts or 10 ng to 100 ng gdna. Sybr green i dye amplitaq fast dna polymerase up ultra pure uracil dna glycosylase udg.
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